Accession Number : ADP019886
Title : Lanthanide Enhanced Luminescence (LEL) with One and Two Photon Excitation of Quantum Dyes(copyright) Lanthanide(III)-Macrocycles
Descriptive Note : Conference paper
Corporate Author : VIRGINIA COMMONWEALTH UNIV RICHMOND DEPT OF CHEMISTRY
Personal Author(s) : Leif, Robert C. ; Becker, Margie C. ; Bromm Jr., Al ; Chen, Nanguang ; Cowan, Ann E. ; Vallarino, Lidia M. ; Yang, Sean ; Zucker, Robert M.
Report Date : JAN 2004
Pagination or Media Count : 13
Abstract : Improvements in the lanthanide enhanced luminescence (LEL) protocol have facilitated the use of the recently synthesized Eu(III)-macrocycle-mono-isothiocyanate, Quantum Dye(Registered), as a label. It was discovered that a homogeneous solution in ethanol or other solvent could be used to produce the lanthanide enhanced luminescence (LEL) effect, provided that the solution was permitted to evaporate. This protocol has been applied to the direct staining of cells in S phase, and was optimized for solid phase assays with Quantum Dye labeled streptavidin. Preliminary studies indicate that cells stained with the europium Quantum Dye can be observed both by conventional UV laser excitation and by infrared two-photon confocal microscopy. An enhancer has been found that enables the observation of simultaneous emissions from both the europium and terbium Quantum Dyes.
Descriptors : *QUANTUM THEORY , *PHOTONS , *DYES , *LUMINESCENCE , SYMPOSIA , MICROSCOPY , CELLS(BIOLOGY) , RARE EARTH ELEMENTS , SOLID PHASES , EUROPIUM , TERBIUM , BIOLOGICAL STAINS.
Subject Categories : INORGANIC CHEMISTRY
Distribution Statement : APPROVED FOR PUBLIC RELEASE