Accession Number : ADA626852


Title :   Laboratory Validation of the Sand Fly Fever Virus Antigen Assay


Descriptive Note : Journal article


Corporate Author : SCHOOL OF AEROSPACE MEDICINE WRIGHT- PATTERSON AFB OH PREVENTIVE MEDICINE AND PUBLIC HEALTH DEPT


Personal Author(s) : Reeves, Will K ; Szymczak, Mitchell S ; Burkhalter, Kristen L ; Miller, Myrna M


Full Text : http://www.dtic.mil/dtic/tr/fulltext/u2/a626852.pdf


Report Date : Dec 2015


Pagination or Media Count : 5


Abstract : Sandfly fever group viruses in the genus Phlebovirus (family Bunyaviridae) are widely distributed across the globe and are a cause of disease in military troops and indigenous peoples. We assessed the laboratory sensitivity and specificity of the Sand Fly Fever Virus Antigen Assay, a rapid dipstick assay designed to detect sandfly fever Naples virus (SFNV) and Toscana virus (TOSV) against a panel of phleboviruses. The assay detected SFNV and TOSV, as well as other phleboviruses including Aguacate, Anahanga, Arumowot, Chagres, and Punta Toro viruses. It did not detect sandfly fever Sicilian, Heartland, Rio Grande, or Rift Valley fever viruses. It did not produce false positive results in the presence of uninfected sand flies (Lutzomyia longipalpis) or Cache Valley virus, a distantly related bunyavirus. Results from this laboratory evaluation suggest that this assay may be used as a rapid field-deployable assay to detect sand flies infected with TOSV and SFNV, as well as an assortment of other phleboviruses.


Descriptors :   *SANDFLY FEVER VIRUS INFECTION , ASSAYING , DIPTERA , DISEASES , DISTRIBUTION , FEVERS , LABORATORIES , LABORATORY TESTS , MEASURING INSTRUMENTS , MILITARY PERSONNEL , REPRINTS , RIFT VALLEY FEVER , SENSITIVITY , VIRUSES


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE