Accession Number : ADA623459


Title :   Measurement of Metabolic Activity in Dormant Spores of Bacillus Species


Descriptive Note : Final rept. 1 Apr-31 Dec 2014


Corporate Author : CONNECTICUT UNIV HEALTH CENTER FARMINGTON


Personal Author(s) : Setlow, Peter


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA623459


Report Date : 14 Jan 2015


Pagination or Media Count : 7


Abstract : Spores of Bacillus megaterium and Bacillus subtilis were harvested shortly after release from sporangia, incubated under various conditions and spore metabolism monitored by 31P-NMR of small molecules. Incubation for up to 30 d at 4-50 Degrees C in water or buffer to raise spore core pH to 7.8, or at 4 in spent sporulation medium led to no changes in small molecules including 3-phosphoglyceric acid (3PGA) or mono nucleotides, and no ATP accumulation. Similar results were obtained with spores that had gone through Stage I of germination and were incubated 8 d at 37 Degrees C. However, spores incubated in spent sporulation medium at 37 or 50 degraded much 3PGA and accumulated mono nucleotides (but not ATP!), and these processes were accelerated when core pH was raised to 7.8. These data indicate that spores store in water or buffer at low or high temperatures exhibit little if any metabolism of endogenous compounds. However, spores stored in spent sporulation medium did exhibit some metabolism, including 3PGA degradation and mono nucleotide accumulation, the latter indicative of RNA degradation. These results indicate that dormant spores can carry out some metabolic reactions.


Descriptors :   *BACILLUS SUBTILIS , *DORMANCY , *GERMINATION , *METABOLISM , *SPORES , ACCUMULATION , BACTERIOLOGY , BUFFERS , CHEMICAL REACTIONS , DEGRADATION , GLYCERIDES , HIGH TEMPERATURE , INCUBATION , MOLECULAR DYNAMICS , NUCLEOTIDES , PH FACTOR , RECEPTOR SITES(PHYSIOLOGY) , RIBONUCLEIC ACIDS , SPORULATION , TRANSCRIPTION(GENETICS) , WATER


Subject Categories : Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE