Accession Number : ADA623360


Title :   Architecture and Assembly of the Bacillus subtilis Spore Coat


Descriptive Note : Journal article


Corporate Author : CONNECTICUT UNIV HEALTH CENTER FARMINGTON


Personal Author(s) : Plomp, Marco ; Carroll, Alicia M ; Setlow, Peter ; Malkin, Alexander J


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA623360


Report Date : 26 Sep 2014


Pagination or Media Count : 19


Abstract : In this study, atomic force microscopy was used to probe the coat structure of Bacillus subtilis wild-type and cotA, cotB, safA, cotH, cotO, cotE, gerE and cotE gerE spores. This approach provided high-resolution visualization of the various coat structures, new insight into the function of specific coat proteins, enabled the development of a detailed model of spore coat architecture, and identified several spore coat layers that have not previously been observed. This work also provided evidence that the spores' inner coat is crystalline, with its apparent two-dimensional (2D) nuclei being the first example of a non-mineral 2D crystallization pattern in a biological organism.


Descriptors :   *ASSEMBLY , *BACILLUS SUBTILIS , *SPORES , AMORPHOUS MATERIALS , ARCHITECTURE , ATOMIC SPECTROSCOPY , BACTERIA , BIOLOGICAL DETECTION , CELLS(BIOLOGY) , COATINGS , CRYSTALLIZATION , ELECTRON MICROSCOPY , ENZYMES , FIBERS , GERMINATION , HIGH RESOLUTION , HONEYCOMB STRUCTURES , MOLECULAR DYNAMICS , NANOPARTICLES , NUCLEATION , PATTERNS , PROTEINS , STRAINS(BIOLOGY)


Subject Categories : Biochemistry
      Microbiology
      Crystallography
      Atomic and Molecular Physics and Spectroscopy


Distribution Statement : APPROVED FOR PUBLIC RELEASE