Accession Number : ADA614115


Title :   Cell of Origin: Exploring an Alternative Contributor to Ovarian Cancer


Descriptive Note : Annual rept. 1 Sep 2013-31 Aug 2014


Corporate Author : MASSACHUSETTS GENERAL HOSPITAL BOSTON


Personal Author(s) : Rueda, Bo R


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA614115


Report Date : Sep 2014


Pagination or Media Count : 15


Abstract : Our studies to date have determined that human oogonial stem cells, while far less stable than their murine counterparts, can be successfully expanded and propagated in culture. This has allowed us to begin testing the potential tumorigenicity of these cells with the ultimate goal of comparing the DNA signature of the oogonial stem cell-derived tumors to that of primary human ovarian cancer. We have also successfully introduced in human oogonial stem cells genetic alterations commonly detected in ovarian cancer. We are now generating tumors from these altered oogonial stem cells and will compare the histologic features in the tumors formed from the modified oogonial stem cells to those of primary tumors collected from women diagnosed with serous ovarian cancer. Also of importance is the identification of Ddx4-positive cells in xenografts derived from primary human ovarian serous tumors. The biological significance of this apparent rare population is yet to be determined. We have designed and implemented initial studies to test the relative tumorigenicity of Ddx4-positive, CD133-positive and Ddx4 CD133 double positive fractions. Demonstrating that these highly specialized human oogonial stem cells have the capacity to form ovarian tumors would be a major paradigm shift.


Descriptors :   *DEOXYRIBONUCLEIC ACIDS , *OVARIAN CANCER , *STEM CELLS , COMPARISON , DIAGNOSIS(MEDICINE) , HISTOLOGY , NEOPLASMS


Subject Categories : Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE