Accession Number : ADA593678


Title :   Exploring Neurofibromin Function in a Yeast Model of NF1


Descriptive Note : Revised final rept. 1 Sep 2008-31 Aug 2011


Corporate Author : PENNSYLVANIA UNIV PHILADELPHIA


Personal Author(s) : Gitler, Aaron


Full Text : http://www.dtic.mil/dtic/tr/fulltext/u2/a593678.pdf


Report Date : Nov 2011


Pagination or Media Count : 14


Abstract : We hypothesized that the NF1 protein Neurofibromin has functions in addition to its Ras-GAP activity and proposed to develop a yeast NF1 model to define new functions as well as novel regulators of known functions. In this 3 year project, we categorized genetic hits that we recovered from yeast screens. We divided hits into Ras-dependent and Ras-independent categories. We have identified proteins that physically interact with IRA1 and IRA2 (the yeast homologs of human Neurofibromin). We have also focused on a novel genetic interaction between IRA1 and IRA2 and yeast genes involved in peroxisome formation and function in yeast. These studies will illuminate the cellular pathways that Ira1 and Ira2 regulate and how neurofibromin deficiency contributes to disease.


Descriptors :   *FIBROSIS , *YEASTS , DISEASES , GENES , GENETICS , INTERACTIONS , MASS SPECTROMETRY , PURIFICATION


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE