Accession Number : ADA570546


Title :   Role of Mitochondrial Inheritance on Prostate Cancer Outcome in African American Men


Descriptive Note : Annual rept. 30 Sep 2011-29 Sep 2012


Corporate Author : BAYLOR COLL OF MEDICINE HOUSTON TX


Personal Author(s) : Mims, Martha


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA570546


Report Date : Oct 2012


Pagination or Media Count : 10


Abstract : We are examining the hypothesis that mitochondrial inheritance plays a significant role in aggressiveness of prostate cancer in African Americans. In the first year of the project we have identified 2,000 noncancerous tissues samples from African American men with prostate cancer and we have extracted DNA from 1,500 of them to date. We have validated a robust new DNA sequencing technique developed by our collaborator using single amplicon long-range PCR that permits deep coverage (10,000-20,000X on average) of the mitochondrial genome. We have more than 300 samples in the sequencing pipeline with several hundred more to be submitted in the next few weeks. We have begun mapping of DNA variants in our sequenced genomes to mitochondrial genes. Illumina African Admixture mapping of the initial 72 subjects has been accomplished. Initial steps toward creating the Rho0 cell lines necessary for cybrid generation have begun with generation of puromycin resistant cell lines and testing of two different technologies for eliminating mitochondria from the nuclear donor cell lines.


Descriptors :   *PROSTATE CANCER , AFRICAN AMERICANS , DEOXYRIBONUCLEIC ACIDS , GENOME , MITOCHONDRIA , TISSUES(BIOLOGY)


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE