Accession Number : ADA560603


Title :   Epithelial and Stromal Spectral Imaging for Rapid Surgical Margin Analysis


Descriptive Note : Annual summary rept. 1 Mar 2011-28 Feb 2012


Corporate Author : DARTMOUTH COLL HANOVER NH


Personal Author(s) : Laughney, Ashley ; Krishnaswamy, Venkataramanan ; Paulsen, Keith ; Pogue, Brian


Full Text : http://www.dtic.mil/dtic/tr/fulltext/u2/a560603.pdf


Report Date : Mar 2012


Pagination or Media Count : 18


Abstract : A new, scanning beam spectroscopy platform was developed to dramatically improved image acquisition speed of localized light scattering. The new scanning in situ spectroscopy platform samples broadband reflectance from a 150 microns diameter spot over a 1 sq cm field using a dark field geometry and telecentric lens; the system was designed to balance sensitivity to stromal and epithelial distributions, while sufficiently imaging the inherent diversity within a diagnosis. The diagnostic performance of this new imaging system was tested in 29 tissues procured during breast conservative surgery. Nearly 300,000 broadband spectra were parameterized using light scattering models and spatially dependent spectra signatures were interpreted using co-occurrence matrix representation of image texture. Local scattering changes distinguished benign from malignant pathologies with 94% accuracy, 85% sensitivity, 100% specificity, and 100% positive & 90% negative predictive value, using a threshold-based classifier. Texture and shape features offered unique information about scattering ultra-structures, and suggest a potential contrast mechanism for ductal carcinoma in situ. Images of localized scattering readily identify benign and malignant pathologies in resected specimens and offer new spectral-spatial signatures of clinically relevant breast pathologies.


Descriptors :   *BREAST CANCER , *DIAGNOSIS(MEDICINE) , *EPITHELIUM , ACQUISITION , CANCER , GEOMETRY , IMAGES , LENSES , LIGHT SCATTERING , MAMMARY GLANDS , PATHOLOGY , PATIENTS , SPECTROSCOPY , SURGERY , TEXTURE , TISSUES(BIOLOGY)


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE