Accession Number : ADA558413


Title :   New Advanced Technologies In Stem Cell Therapy


Descriptive Note : Annual rept. 1 Sep 2010-31 Aug 2011


Corporate Author : PITTSBURGH UNIV PA


Personal Author(s) : Huard, Johnny


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA558413


Report Date : Sep 2011


Pagination or Media Count : 52


Abstract : We have isolated and characterized a population of skeletal muscle-derived stem cells (MDSCs) that display a greatly improved skeletal and cardiac muscle transplantation capacity when compared to skeletal muscle myoblasts. The MDSCs ability to withstand oxidative and inflammatory stresses appears to be the single most important factor for their improved transplantation capacity. Although the true origin of MDSCs remains unclear, their high degree of similarity with blood vessel-derived stem cells suggests their potential origin could be from the vascular wall. We have recently isolated two distinct populations of cells from the vasculature of human skeletal muscle known collectively as human skeletal muscle-derived cells (hMDCs). The two populations are myo-endothelial cells and pericytes and both can repair skeletal and cardiac muscles in a more effective manner than myoblasts, as is observed with murine MDSCs. In the current proposal we intend to evaluate and compare the regeneration capacity of these two hMDC populations after their implantation into the skeletal muscle of immunodeficient/dystrophic (SCID/mdx) mice. We will then investigate the influence that sex has on the regeneration and repair capacity of the hMDCs endowed with the greatest regeneration capacity (either myo-entothelial cells or pericytes). Finally we will investigate the influence that age plays on the regeneration capacity of the cells.


Descriptors :   *MUSCLES , *STEM CELLS , *TRANSPLANTATION , BLOOD , CAPACITY(QUANTITY) , CARDIOVASCULAR SYSTEM , HEART , HUMANS , IMPLANTATION , INFLAMMATION , ISOLATION , MUSCULOSKELETAL SYSTEM , OXIDATION , POPULATION , REGENERATION(ENGINEERING) , SKELETON , STRESSES


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE