Accession Number : ADA512469


Title :   Affinity Probe Capillary Electrophoresis Evaluation of Aptamer Binding to Campylobacter jejuni Bacteria


Descriptive Note : Progress rept. 2007-2008


Corporate Author : ARMY RESEARCH LAB ADELPHI MD


Personal Author(s) : Stratis-Cullum, Dimitra N. ; McMasters, Sun ; Pellegrino, Paul M.


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA512469


Report Date : NOV 2009


Pagination or Media Count : 22


Abstract : In this work, we developed a capillary electrophoresis immunoassay to evaluate aptamer binding to bacterial cells. This work is the first known demonstration of aptamer affinity probe capillary electrophoresis to a whole cell target. The aptamer probes investigated were developed against a Campylobacter jejuni bacterial cell target, and we evaluated binding relative to other common food-borne pathogens. Specific binding affinity was evidenced by a pronounced mobility shift and peak broadening with increasing bacteria concentration. Little to no mobility shift was observed for food-borne pathogens, Salmonella typhirium and Escherichia coli, even when increasing concentrations 10-fold over target. These results suggest that affinity probe capillary electrophoresis could be useful for qualitative screening of aptamer candidates to bacterial cell targets.


Descriptors :   *PATHOGENIC MICROORGANISMS , *JEJUNUM , *ESCHERICHIA COLI , IMMUNOASSAY , FOOD , BIOASSAY , ELECTROPHORESIS , SALMONELLA , SHIFTING , CAPILLARITY , BACTERIA


Subject Categories : MEDICINE AND MEDICAL RESEARCH
      MICROBIOLOGY


Distribution Statement : APPROVED FOR PUBLIC RELEASE