Accession Number : ADA512469
Title : Affinity Probe Capillary Electrophoresis Evaluation of Aptamer Binding to Campylobacter jejuni Bacteria
Descriptive Note : Progress rept. 2007-2008
Corporate Author : ARMY RESEARCH LAB ADELPHI MD
Personal Author(s) : Stratis-Cullum, Dimitra N. ; McMasters, Sun ; Pellegrino, Paul M.
Report Date : NOV 2009
Pagination or Media Count : 22
Abstract : In this work, we developed a capillary electrophoresis immunoassay to evaluate aptamer binding to bacterial cells. This work is the first known demonstration of aptamer affinity probe capillary electrophoresis to a whole cell target. The aptamer probes investigated were developed against a Campylobacter jejuni bacterial cell target, and we evaluated binding relative to other common food-borne pathogens. Specific binding affinity was evidenced by a pronounced mobility shift and peak broadening with increasing bacteria concentration. Little to no mobility shift was observed for food-borne pathogens, Salmonella typhirium and Escherichia coli, even when increasing concentrations 10-fold over target. These results suggest that affinity probe capillary electrophoresis could be useful for qualitative screening of aptamer candidates to bacterial cell targets.
Descriptors : *PATHOGENIC MICROORGANISMS , *JEJUNUM , *ESCHERICHIA COLI , IMMUNOASSAY , FOOD , BIOASSAY , ELECTROPHORESIS , SALMONELLA , SHIFTING , CAPILLARITY , BACTERIA
Subject Categories : MEDICINE AND MEDICAL RESEARCH
Distribution Statement : APPROVED FOR PUBLIC RELEASE