Accession Number : ADA507006


Title :   Developmental and Environmental Regulation of AaeIAP1 Transcript in Aedes aegypti


Descriptive Note : Journal article


Corporate Author : DEPARTMENT OF AGRICULTURE GAINESVILLE FL


Personal Author(s) : Pridgeon, Julia W ; Zhao, Liming ; Becnel, James J ; Clark, Gary G ; Linthicum, Kenneth J


Full Text : http://www.dtic.mil/dtic/tr/fulltext/u2/a507006.pdf


Report Date : Jan 2008


Pagination or Media Count : 10


Abstract : Apoptosis (programmed cell death) is a tightly regulated physiological process. The inhibitors of apoptosis proteins (IAPs) are key regulators for apoptosis. An inhibitor of apoptosis protein gene IAP1 was recently cloned from Aedes aegypti (L.) (AaeIAP1, GenBank accession no. DQ993355); however, it is not clear whether AaeIAP1 is developmentally and environmentally regulated. In this study, we applied quantitative polymerase chain reaction (PCR) to investigate the expression levels of the AaeIAP1 transcript in different developmental stages and under different environmental conditions. Our results revealed that the expression of the AaeIAP1 transcript was detectable in all life stages of Ae. aegypti, with significantly higher levels in pupal and adult stages than in larval stages. Furthermore, when Ae. aegypti was exposed to all stressful environmental conditions (e.g., low and high temperatures, UV radiation, acetone, and permethrin insecticide treatment), the expression level of AaeIAP1 transcript was increased significantly. Our results suggest that AaeIAP1 might play an important role in both the physiological development of Ae. aegypti and stress-induced apoptosis.


Descriptors :   *INFECTIOUS DISEASES , *AEDES , *LARVAE , *CELLS(BIOLOGY) , *APOPTOSIS , CLONES , ACETONES , DEATH , TRANSCRIPTION(GENETICS) , REPRINTS , COMPUTER PROGRAMMING , PROTEINS , ULTRAVIOLET RADIATION , VERTEBRATES


Subject Categories : Medicine and Medical Research
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE