Accession Number : ADA480360


Title :   Identification of the Transformational Properties and Transcriptional Targets of the Oncogenic SRY Transcription Factor SOX4


Descriptive Note : Annual summary, 11 Dec 2006-10 Dec 2007


Corporate Author : EMORY UNIV ATLANTA GA


Personal Author(s) : Scharer, Christopher


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA480360


Report Date : JAN 2008


Pagination or Media Count : 14


Abstract : The exact role of SOX4 in development and promoting tumorigenesis however is currently unknown. Here we sought to identify the direct transcriptional targets of SOX4 on a global scale to determine the gene networks affected in human cancers and development. Using chromatin immunoprecipitation coupled to DNA microarrays tiling the promoters of 25,000 known genes (ChIP-chip), we identified 140 high confidence promoter regions bound by SOX4 in living human prostate cancer cells. We have also used a unique protein-binding double-stranded DNA microarray to determine a novel SOX4 specific position-weight matrix for in silico SOX4 binding site searches. Direct targets of SOX4 include several key cellular regulators and 11 other transcription factors such as SOX11, ZNF281, and ZHX2. SOX4 impacts the Notch pathway, FGF signaling via regulation of FGFRL1, as well as the Hedgehog pathway via regulation GLIS2. These data provide new insights into how SOX4 impacts growth factor and developmental signaling pathways and how these changes may influence cancer progression and development.


Descriptors :   *TRANSCRIPTION(GENETICS) , *PROSTATE CANCER , *DEOXYRIBONUCLEIC ACIDS , *ONCOGENESIS , CHEMICAL PRECIPITATION , CELLS(BIOLOGY) , IMMUNOASSAY , CANCER , GROWTH(PHYSIOLOGY) , CHROMATIN , GENES , REGULATORS , NEOPLASMS , NETWORKS , HUMANS


Subject Categories : BIOCHEMISRTY
      MEDICINE AND MEDICAL RESEARCH


Distribution Statement : APPROVED FOR PUBLIC RELEASE