Accession Number : ADA469803


Title :   A Novel Combination of Thermal Ablation and Heat-Inducible Gene Therapy for Breast Cancer Treatment


Descriptive Note : Annual summary rept. 31 Mar 2006-30 Mar 2007


Corporate Author : DUKE UNIV DURHAM NC


Personal Author(s) : Liu, Yunbo ; Zhong, Pei


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA469803


Report Date : APR 2007


Pagination or Media Count : 6


Abstract : This investigation aims to test the hypothesis that HIFU not only can destroy primary tumor tissues by thermal ablation but also is capable of inducing therapeutic effects in sub-lethally injured tumor cells via the heat shock response. During the current funding period, we have fully developed an image-guided, computer-controlled experimental HIFU system and characterized its corresponding acoustic and thermal fields. The efficiency of HIFU-induced maker gene (GFP) activation under the control of hsp70B promoter was investigated in vitro by using a mice breast cancer cell line (4T1). Furthermore, a 3D cell-embedded tissue mimicking phantom was developed, which possesses similar acoustic and thermal properties to that of breast tissues. Using this 3D phantom model, we have performed preliminary experiments to determine the correlation between the spatial thermal dose distribution and resultant gene activation during HIFU treatment. Future work will focus on completion of the HIFU-induced gene activation in the 3-D tissue mimicking phantom and on gene activation during HIFU thermal ablation in vivo.


Descriptors :   *GENES , *HEAT TOLERANCE , *BREAST CANCER , THERMAL PROPERTIES , ABLATION , SPATIAL DISTRIBUTION , TISSUES(BIOLOGY) , NEOPLASMS , RESPONSE(BIOLOGY) , IN VIVO ANALYSIS , MAMMARY GLANDS , ACOUSTIC FIELDS , HEAT , HYPOTHESES , IN VITRO ANALYSIS , SHOCK , DOSAGE , THERAPY


Subject Categories : ANATOMY AND PHYSIOLOGY
      MEDICINE AND MEDICAL RESEARCH
      STRESS PHYSIOLOGY


Distribution Statement : APPROVED FOR PUBLIC RELEASE