Accession Number : ADA466643


Title :   Origin and Properties of Prostatic Stem Cells


Descriptive Note : Final rept. 21 Jan 2004-20 Jan 2007


Corporate Author : NEW YORK UNIV NY SCHOOL OF MEDICINE


Personal Author(s) : Wilson, E. L. ; Gupta, Rashmi


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA466643


Report Date : FEB 2007


Pagination or Media Count : 32


Abstract : The aims of this proposal were to isolate and characterize prostatic epithelial stem cells and also to determine if bone marrow (BM) cells can differentiate into prostatic tissue. We show that the murine prostate contains a small population of cells (<1%) capable of effluxing the Hoechst dye by an active process (the side population, SP), indicating the presence of cells with features of stem cells. As SP cells expressed Sca-1, a protein expressed by stem cells of other origins, we isolated Sca-1 expressing cells and determined their proliferative ability in an in vivo prostate reconstitution assay. We show that prostate stem cells reside in the proximal region of ducts within the population that expresses high levels of Sca-1. We also show that BM stem cells can differentiate into prostatic epithelial and stromal cells. Low numbers of basal epithelial cells (-1%) are of BM origin but significant numbers of stromal cells (-15%) are derived from BM. These findings may have considerable implications for prostatic diseases such as cancer as gastric cancer has been shown to originate in BM-derived cells.


Descriptors :   *BONE MARROW , *STEM CELLS , EPITHELIUM , TISSUES(BIOLOGY) , FLUORESCENT DYES , BIOASSAY , IN VIVO ANALYSIS , CELLS(BIOLOGY) , ANTIGENS


Subject Categories : ANATOMY AND PHYSIOLOGY
      MEDICINE AND MEDICAL RESEARCH


Distribution Statement : APPROVED FOR PUBLIC RELEASE