Accession Number : ADA444662


Title :   Protection Conferred by recombinant Yersinia pestis Antigens Produced by a Rapid and Highly Scalable Plant Expression System


Descriptive Note : Journal article


Corporate Author : ARIZONA STATE UNIV TEMPE


Personal Author(s) : Santi, Luca ; Giritch, Anatoli ; Roy, Chad J. ; Marillonnet, Sylvestre ; Klimyuk, Victor ; Gleba, Yuri ; Webb, Robert ; Arntzen, Charles J. ; Mason, Hugh S.


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA444662


Report Date : 24 JAN 2006


Pagination or Media Count : 7


Abstract : Plague is still an endemic disease in different regions of the world. Increasing reports of incidence, the discovery of antibiotic resistance strains, and concern about a potential use of the causative bacteria Yersinia pestis as an agent of biological warfare have highlighted the need for a safe, efficacious, and rapidly producible vaccine. The use of F1 and V antigens and the derived protein fusion F1-V has shown great potential as a protective vaccine in animal studies. Plants have been extensively studied for the production of pharmaceutical proteins as an inexpensive and scalable alternative to common expression systems. In the current study the recombinant plague antigens F1, V, and fusion protein F1-V were produced by transient expression in Nicotiana benthamiana by using a deconstructed tobacco mosaic virus-based system that allowed very rapid and extremely high levels of expression. All of the plant-derived purified antigens, administered s.c. to guinea pigs, generated systemic immune responses and provided protection against an aerosol challenge of virulent Y. pestis.


Descriptors :   *ANIMALS , *ANTIGENS , *PLANTS(BOTANY) , *YERSINIA PESTIS , *VACCINES , TRANSIENTS , AEROSOLS , BACTERIA , PROTEINS , DISEASES , PLAGUES , GUINEA PIGS , DRUGS , IMMUNITY , RESPONSE(BIOLOGY) , PARALLEL PROCESSING , STRAINS(BIOLOGY) , SCALING FACTOR , BIOLOGICAL WARFARE


Subject Categories : BIOLOGY
      MEDICINE AND MEDICAL RESEARCH
      MICROBIOLOGY


Distribution Statement : APPROVED FOR PUBLIC RELEASE