Accession Number : ADA436905


Title :   Genome-Wide Chromosomal Targets of Oncogenic Transcription Factors


Descriptive Note : Annual rept. 31 Mar 2004-30 Mar 2005


Corporate Author : TEXAS UNIV AT AUSTIN


Personal Author(s) : Iyer, Vishwanath R.


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA436905


Report Date : APR 2005


Pagination or Media Count : 17


Abstract : The overall objective of this Idea Award was to develop a novel method for identifying the direct transcriptional targets of oncogenic transcription factors that are important in breast cancer. We have developed STAGE (Sequence Tag Analysis of Genomic Enrichment) as a novel genome-wide method for identifying the chromosomal targets of DNA binding proteins in any sequenced genome. STAGE is based on high-throughput sequencing of concatamerized tags derived from DNA associated with transcription factors that is isolated by chromatin immunoprecipitation. The application of STAGE to oncogenic transcription factors involved in breast cancer can help elucidate their role in gene regulatory networks underlying the disease. We have used STAGE to identify several novel targets of E2F4 in human cells. E2F4 is a transcription factor that is a member of the E2F family of transcription factors that is important in cell proliferation. We have verified many of the novel targets we predicted by STAGE using independent means such as promoter specific PCR and ChIP-chip. We have successfully carried out chromatin immunoprecipitation for c-myc and ER in human cells and are currently analyzing those samples using STAGE.


Descriptors :   *CHROMOSOMES , *BREAST CANCER , *TRANSCRIPTION(GENETICS) , PROTEINS , MEDICAL RESEARCH , ONCOGENESIS , GENOME , CHROMATIN , CELLS(BIOLOGY) , DEOXYRIBONUCLEIC ACIDS.


Subject Categories : GENETIC ENGINEERING AND MOLECULAR BIOLOGY
      MEDICINE AND MEDICAL RESEARCH


Distribution Statement : APPROVED FOR PUBLIC RELEASE