Accession Number : ADA436423


Title :   Modeling Human Epithelial Ovarian Cancer in Mice by Alteration of Expression of the BRCA1 and/or P53 Genes


Descriptive Note : Annual rept. 1 Feb 2004-31 Jan 2005


Corporate Author : FOX CHASE CANCER CENTER PHILADELPHIA PA


Personal Author(s) : Connolly, Denise C.


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA436423


Report Date : FEB 2005


Pagination or Media Count : 10


Abstract : About 1 out of every 10 cases of epithelial ovarian cancer is inherited. Unlike non-hereditary (sporadic) ovarian cancer, some of the underlying genetic causes of hereditary ovarian cancer are well understood. The majority, more than 90%, of inherited cases are the result of inherited mutations in the breast cancer associated gene 1 (BRCA1). In addition to mutations of BRCA1, mutations of the p53 gene are often found in patients with breast and ovarian cancer syndrome. Based on the importance of both of these genes in the development of this type of ovarian cancer, the authors hypothesize that inactivation of BRCA1 and p53 in the ovaries of mice will result in epithelial ovarian cancer in the animals. They have obtained mouse strains that conditionally express the BRCA1 and p53 genes. Tissue-restricted exposure to cre recombinase results in excision of LoxP flanked (floxed) sequences in these mice and expression of the mutated forms of BRCA1 and p53. To achieve cre recombinase expression that is restricted to the ovary, they have employed localized delivery of Adenovirus-Cre by intrabursal injection of virus. They also have developed transgenic mice that should express cre recombinase in a reproductive tissue-restricted and drug inducible manner.


Descriptors :   *EPITHELIUM , *MUTATIONS , *OVARIAN CANCER , *GENES , MICE , ONCOGENESIS , MEDICAL RESEARCH , TRANSCRIPTION(GENETICS) , ONCOGENIC VIRUSES.


Subject Categories : GENETIC ENGINEERING AND MOLECULAR BIOLOGY
      MEDICINE AND MEDICAL RESEARCH


Distribution Statement : APPROVED FOR PUBLIC RELEASE