Accession Number : ADA261892


Title :   Comparative Infectivity Determinations of Dengue Virus Vaccine Candidates in Rhesus Monkeys, Mosquitoes, and Cell Cultures


Descriptive Note : Final rept. 1 Sep 1989-31 Dec 1992


Corporate Author : PUERTO RICO UNIV SAN JUAN


Personal Author(s) : Kraiselburd, Edmundo


Full Text : http://www.dtic.mil/dtic/tr/fulltext/u2/a261892.pdf


Report Date : 28 Jan 1993


Pagination or Media Count : 20


Abstract : Attenuation of virulent viruses are widely used for the development of viral vaccines through the years. These vaccines are made by serial passages of virus in cells from non-natural hosts. The first intents for the development of Dengue virus vaccines was mad by serial intracerebral passages of virus in infant of newly-weaned mice. Primary canine kidney (PCK) cell culture are also used for the attenuation of wild Dengue virus strains. Studies using these attenuated virus strains have been recently published. The objective of this contract was to determine the antibody response of rhesus macaques inoculated with different attenuated dengue virus preparations. Vaccine immunogenecity was determined by the plaque reduction neutralization test (PRNT). The PRNT titer of each serum sample was determined as described elsewhere.


Descriptors :   *VACCINES , *DENGUE , ANTIBODIES , DENGUE VIRUS , MACAQUE MONKEYS , MEDICAL RESEARCH , VIRULENCE


Subject Categories : Medicine and Medical Research
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE