Accession Number : ADA256049


Title :   Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Plasmodium vivax-VK247 Sporozoites


Descriptive Note : Journal article


Corporate Author : WALTER REED ARMY INST OF RESEARCH WASHINGTON DC


Personal Author(s) : Wirtz, Robert A ; Sattabongkot, Jetsumon ; Hall, Ted ; Burkot, Thomas R ; Rosenberg, Ronald


Full Text : http://www.dtic.mil/dtic/tr/fulltext/u2/a256049.pdf


Report Date : Jan 1992


Pagination or Media Count : 5


Abstract : An enzymed-linked immunosorbent assay (ELISA) for the Plasmodium vivax-VK247 (variant) circumsporozoite (CS) protein was developed and evaluated using sporozoites produced by feeding mosquitoes on Thai patients with parasitologically confirmed P. vivax infections. The ELISA had a detection threshold of fewer than 50 sporozoites. Using this assay in conjunction with an ELISA for the VK210 polymorph, nearly 16% of the 235 P. vivax cases produced sporozoites positive only for the variant; 69% produced sporozoites positive only in the VK210 assay; and 15% were positive in both assays, indicating mixed infections. Twelve cases (5%) produced sporozoites negative-in one assay and with unexpectedly low activity in the other ELISA, indicating the possibility of other CS protein polymorphs. Plasmodium vivax, circumsporozoites protein detection ELISA.


Descriptors :   *SPOROZOITES , *PLASMODIUM VIVAX , REPRINTS , DETECTION , ENZYMES , CULICIDAE , PLASMODIUM , FEEDING , PROTEINS , PARASITES


Subject Categories : Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE