Accession Number : ADA238790
Title : Free Radical Mechanisms of Xenobiotic Mammalian Cytotoxicities
Descriptive Note : Final rept.,
Corporate Author : GEORGE WASHINGTON UNIV MEDICAL CENTER WASHINGTON DC DEPT OF MEDICINE
Personal Author(s) : Dickens, Benjamin F.
Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA238790
Report Date : 30 JUN 1991
Pagination or Media Count : 38
Abstract : Our initial goal was to identify if free radical mechanisms are involved in the cytotoxicity of a number of IRP volume I and II chemicals. We found that a number of these agents act to enhance membrane lipid peroxidation in response to a standard dose of exogenous free radicals. Using chlorinated hydrocarbons (carbon tetrachloride, trichloroethylene, dichloroethylene, trichloroethane, dichloroethane) as a model for other IRP chemicals, we established conditions to measure lipid peroxidation in cultured smooth muscle and endothelial cells. These agents induced lipid peroxidation in the presence of physiological levels of iron in these vascular cells by a mechanism that doesn't require cytochrome P-450. Antiradical treatment with deferoxamine and probucol (but not SOD, catalase, or mannitol) appear to reduce the toxicity of these agents.
Descriptors : *MEASUREMENT , MEMBRANES(BIOLOGY) , CHEMICALS , CELLS , TOXICITY , LIPIDS , IRON , OXIDATION , DOSAGE , PHYSIOLOGY , MUSCLES , FREE RADICALS , CELLS(BIOLOGY) , CARDIOVASCULAR SYSTEM , CHLORINATED HYDROCARBONS , TRICHLOROETHYLENE , TRICHLOROETHANES , ENDOTHELIUM , CHLOROETHANES , CARBON TETRACHLORIDE , CATALASE , MANNITOL , VOLUME
Subject Categories : MEDICINE AND MEDICAL RESEARCH
Distribution Statement : APPROVED FOR PUBLIC RELEASE