Accession Number : ADA231385


Title :   Determination of Dimethyl Sulfoxide (DMSO), Ethanol (ETOH), Formamide (F) and Glycerol/Formal (GF) by High Performance Liquid Chromatography (HPLC)


Descriptive Note : Technical rept. Jan-May 1988


Corporate Author : ARMY BIOMEDICAL RESEARCH AND DEVELOPMENT LAB FORT DETRICK MD


Personal Author(s) : Brueggemann, Ernst E. ; Rosencrance, Alan B.


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA231385


Report Date : 30 JAN 1989


Pagination or Media Count : 17


Abstract : Liquid chromatographic (LC) methods have been developed for the determination of dimethyl sulfoxide (DMSO), ethanol (ETOH), formamide (F), and glycerol/formal (GF), in aqueous samples. The samples are directly injected onto a high performance liquid chromatograph (HPLC) without further sample preparation. Separation of DMSO and F was achieved by using a C18 column and a mobile phase containing water/methanol. Separation of GS and ETOH was achieved by using a Dextro-Pak column and a mobile phase consisting of water. A programmable ultraviolet (UV) spectrophotometric detector was used to monitor the LC effluent for DMSO and F. A refractive index detector was used to monitor the LC effluent for ETOH and GF. The precision and accuracy of all methods are included in the report.


Descriptors :   *DETECTORS , WATER , COMPUTER PROGRAMMING , REFRACTIVE INDEX , ACCURACY , HIGH RESOLUTION , METHANOLS , MOBILE , LIQUID CHROMATOGRAPHY , PRECISION , SAMPLING , DETERMINATION , GLYCEROLS , METHYL SULFOXIDE , SPECTROPHOTOMETRY , ULTRAVIOLET RADIATION


Subject Categories : PHYSICAL CHEMISTRY


Distribution Statement : APPROVED FOR PUBLIC RELEASE