Accession Number : ADA227293
Title : Detection of Rickettsiae in Arthropod Vectors by DNA Amplification Using the Polymerase Chain Reaction
Descriptive Note : Journal article
Corporate Author : NAVAL MEDICAL RESEARCH INST BETHESDA MD
Personal Author(s) : Azad, Abdu F. ; Webb, Laura ; Carl, Mitchell ; Dasch, Gregory A.
Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA227293
Report Date : 1990
Pagination or Media Count : 8
Abstract : Polymerase chain reaction (PCR) amplification of DNA was used to detect Rickettsia rickettsii and R. typhi in experimentally infected adult Dermacentor variabilis ticks and Xenopsylla cheopis fleas, respectively. A primer pair derived from the 17-kDa-antigen gene sequence of typhus and spotted fever group rickettsiae was used to amplify a 434-base (bp) fragment of the genome of the rickettsiae. The specific PCR-amplified product in extracts of individual infected fleas or ticks was detected readily on ethidium bromide-stained agarose gels. The amplified 434-bp sequence was not detected in uninfected controls. The PCR procedure provides a rapid, sensitive, and highly specific assay for detection of rickettsial infection in arthropod vectors. Keywords: Antigen detection, Indirect immunofluorescence, Rickettsia rickettsii, oligonucleotide primers.
Descriptors : *ARTHROPOD BORNE DISEASES , *RICKETTSIA RICKETTSII , *DEOXYRIBONUCLEIC ACIDS , PRIMERS , AMPLIFICATION , INFECTIOUS DISEASES , TICKS , ASSAYING , ANTIGENS , TYPHUS , DISEASE VECTORS , SIPHONAPTERA , DETECTION , RICKETTSIA , CONTROL , REPRINTS
Subject Categories : BIOLOGY
Distribution Statement : APPROVED FOR PUBLIC RELEASE