Accession Number : ADA211507


Title :   A Method for the Quantitation of Trace Levels of Dimethyl Sulfoxide in Urine by High Performance Liquid Chromatography


Descriptive Note : Suffield rept.


Corporate Author : DEFENCE RESEARCH ESTABLISHMENT SUFFIELD RALSTON (ALBERTA)


Personal Author(s) : Chenier, Claude L. ; Hancock, James R.


Report Date : MAY 1989


Pagination or Media Count : 32


Abstract : According to western intelligence reports, the Eastern Bloc countries are well in the lead in research and development of chemical warfare agents (1- 3). Therefore, the NATO countries are interested in designing, testing, and improving protective equipment (PE), and their associated standard operating procedures (SOPs) as a defence against these agents. One method to evaluate the protection level of the PE and SOPs would be to use, in a realistic fashion, an intake simulant of a chemical warfare agent. Should the intake simulant enter the body and subsequently be detected in the body fluids then this would indicate faulty equipment and/or procedures. A semi-automated liquid chromatographic system for the extraction and concentration of trace levels of dimethyl sulfoxide (DMSO) in human urine has been developed. Urine samples are passed through a low pressure cation-exchange column as a cleanup step prior to solid phase extraction on a low pressure charcoal column. With this method, previously unreported low levels of naturally occurring DMSO were discovered. The method also revealed a decrease in recoveries of DMSO with time indicating a potential instability of the analyte at these low concentrations. Keywords: Chemical warfare agent simulant; Test methods.


Descriptors :   *TEST METHODS , *CHEMICAL AGENT SIMULANTS , INTELLIGENCE , HUMANS , LIQUIDS , CANADA , REPORTS , LOW PRESSURE , HIGH RESOLUTION , LIQUID CHROMATOGRAPHY , SAMPLING , LOW LEVEL , CONCENTRATION(COMPOSITION) , PROTECTION , PROTECTIVE EQUIPMENT , BODY FLUIDS , SEMIAUTOMATIC , CHEMICAL WARFARE AGENTS , URINE , SOLID PHASES , METHYL SULFOXIDE , CHARCOAL , CHROMATOGRAPHS , NATO , STABILITY


Subject Categories : CHEMICAL, BIOLOGICAL AND RADIOLOGICAL WARFARE


Distribution Statement : APPROVED FOR PUBLIC RELEASE