Accession Number : ADA211208


Title :   Effects of Phorbol Esters and Lipopolysaccharide on Endothelial Cell Microfilaments: Laser Scanning Confocal Microscopy and Quantitative Morphometry of Dose Dependent Changes


Descriptive Note : Technical rept.


Corporate Author : NAVAL MEDICAL RESEARCH INST BETHESDA MD


Personal Author(s) : Nielsen, Thor B. ; Wood, David K.


Report Date : 29 NOV 1988


Pagination or Media Count : 22


Abstract : The disruptive action of phorbol esters on microfilament integrity was used to develop a comparison of dosage effects with effects on the modulation of the phosphoinostitide turnover and protein kinase C regulatory system. The novel technique of laser scanning confocal epifluorescence was used to study fiber orientation in phorbol ester treated cells. Dose-dependence of morphological changes was compared and contrasted to the dose-dependent effect of phorbol esters on bradykinin-stimulated phosphoinositide turnover. PB4DB was less potent in inducing the disruption of microfilament structure than in inhibiting phosphoinostide turnover. Lipopolysaccharide was ineffective in inducing a morphological change under these conditions. A simple activation of protein inase C is insufficient to explain the dose-dependent effects of phorbol esters. Thus a morphometric analysis can help distinguish the potency of modulators. Keywords: Morphometric analysis, Endothelial cells; Laser scanning confocal microscopy, Microfilaments; Phorbol ester fluorescence microscopy; Phosphoinositide turnover.


Descriptors :   *ESTERS , *ENDOTHELIUM , *OPTICAL SCANNING , PROTEINS , COMPARISON , MORPHOLOGY , ORIENTATION(DIRECTION) , LASERS , MICROSCOPY , DOSAGE , FILAMENTS , MODULATION , CELLS(BIOLOGY) , MODULATORS , POTENCY , CELLS , FLUORESCENCE , FIBERS , ACTIVATION


Subject Categories : GENETIC ENGINEERING AND MOLECULAR BIOLOGY


Distribution Statement : APPROVED FOR PUBLIC RELEASE