Accession Number : ADA159631


Title :   A Multi-Isotope Procedure for Simultaneously Estimating the Volume of Body Fluid Compartments of Swine.


Descriptive Note : Rept. for Jan 83-May 85,


Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA


Personal Author(s) : Bossone,C A ; Hannon,J P


Full Text : http://www.dtic.mil/dtic/tr/fulltext/u2/a159631.pdf


Report Date : Jun 1985


Pagination or Media Count : 64


Abstract : The purpose of the studies reported in this paper was to develop isotope-based procedures to measure plasma volume, red cell volume, extracellular fluid volume, and total body water individually. Subsequently, techniques would be developed to use a combination of these procedures to measure some or all of the fluid spaces in the pig simulaneously. A procedure was developed for simultaneously estimating the volume of all major body fluid compartments. Splenectomized pigs, 20 to 25 kg, with chronically-implanted carotid artery catheters, received a bolus injection (per kg) (1 uCi 125 I-albumin, 11.7 uCi 51Cr-EDTA, 0.6 uCi 22Na, and 5.3 uCi 3H2O) followed by a 5.2 uCi ml/kg injection of 51 Cr-tagged red cells. Estimated lean body mass averaged 822 g/kg and body fat, 178 g/kg. The new procedure was rapid, technically simple, required small amounts of blood and was applicable to conscious animals.


Descriptors :   *BODY FLUIDS , *RADIOACTIVE ISOTOPES , *TRACER STUDIES , VOLUME , LABORATORY TESTS , TEST METHODS , ESTIMATES , ERYTHROCYTES , BLOOD PLASMA , BLOOD VOLUME , SWINE


Subject Categories : Anatomy and Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE