Accession Number : ADA158414


Title :   Radiometric Methods for Rapid Diagnosis of Viral Infection.


Descriptive Note : Annual rept.,


Corporate Author : JOHNS HOPKINS UNIV BALTIMORE MD


Personal Author(s) : Wagner,H N , Jr ; Tsan,M F ; Charache,P


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA158414


Report Date : Nov 1975


Pagination or Media Count : 18


Abstract : Two radiometric techniques were developed for detecting the presence of herpes simplex virus type 1 in stationary monolayers of the diploid cell line WI-38. The time of detection was compared to that obtained from visual examination for cytopathic effects in the same cell line. Glucose-1-14 oxidation and DNA synthesis of infected and uninfected cells were determined by 14CO2 production measured by an ionization chamber, and 3H-thymidine incorporation measured by scintillation counting, respectively. Infected cells showed a 23 to 26% reduction in glucose-1-14C oxidation and 355 to 498% increase in DNA synthesis four to six hours postinfection as compared to uninfected control cells. These changes in cellular metabolism were observed 14 hours before visible signs of cytopathic effects. The increase in DNA synthesis was completely inhibited by viral neutralization with herpes simplex antiserum. Increased DNA synthesis was observed five hours postinfection with 10,000 to 1,000,001 TCID50 units of virus. The radiometric methods for the detection of viral effect on cellular metabolism are simple, fast, and objective. (Author)


Descriptors :   *DIAGNOSIS(MEDICINE) , *VIRUS DISEASES , LABORATORY TESTS , TISSUE CULTURE CELLS , HUMANS , DEOXYRIBONUCLEIC ACIDS , OXIDATION , LUNG , FIBROBLASTS , INFECTIOUS DISEASES , EMBRYOS , RADIOMETRY , GLUCOSE , RADIOACTIVE ISOTOPES , METABOLIC DISEASES , HERPES SIMPLEX VIRUS , TRITIATED COMPOUNDS , CYTOMEGALOVIRUS , THYMIDINES


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE