Accession Number : ADA108282
Title : Enzymatic Production of Universal Donor Erythrocytes.
Descriptive Note : Final rept. Nov 78-Aug 81,
Corporate Author : CASE WESTERN RESERVE UNIV CLEVELAND OH
Personal Author(s) : Hoskins, Lansing C
Report Date : Oct 1981
Pagination or Media Count : 9
Abstract : The aims of this research contract were: 1) to isolate in pure culture strains of human enteric bacteria with the specialized ability to produce strong activities of extracellular glycosidases that convert blood type A or B erythrocytes to universal donor blood type O erythrocytes; 2) to purify the blood type B-degrading enzyme produced by a fecal strain of Ruminococcus AB; 3) to determine whether human type B red cells could be safety converted by this glycosidase to universal donor type O red cells for use on blood transfusion. Aim 1 was accomplished with the isolation of 2 strains that produce strong blood group A-degrading activity but no B-degrading activity. These complement our previous isolation of the strain of Ruminococcus AB that produces B-degrading but no A-degrading activity. Aim 2, purification of the B-degrading enzyme in culture supernates of Ruminococcus AB, resisted a wide variety of classical protein separation methods until the last 2 months of the contract. As detailed in the report, major purification appears to have been achieved by digestion with papain followed by gel exclusion of chromatography on Sephadex G-200 in 3M NaC1. With successful completion of Aim 2 it will now be possible to meet the objectives of Aim 3.
Descriptors : *ERYTHROCYTES , *BLOOD GROUPS , PRODUCTION , ENZYMES , ISOLATION , PURIFICATION , BLOOD TRANSFUSION , BLOOD ANALYSIS , ENTERIC BACTERIA , BLOOD DONORS , GLYCOSIDASES
Subject Categories : Biochemistry
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE