Accession Number : ADA047797


Title :   Recovery of Alveolar Macrophages from Rhesus and Cynomolgus Monkeys by Lung Lavage.


Descriptive Note : Interim rept.,


Corporate Author : ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD


Personal Author(s) : Kastello,M D ; Emmert,A D ; Denson,R F ; Kishimoto,R A


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA047797


Report Date : 07 Dec 1977


Pagination or Media Count : 17


Abstract : A lung lavage technique has been developed to recover alveolar macrophages from rhesus and cynomolgus monkeys. Sterile saline was injected through an endotracheal tube in anesthetized monkeys and then lung wash fluids containing leukocytes were withdrawn. The lung wash fluids from each monkey routinely contained more than 20 x 10 to the 6th power leukocytes of which 80% were alveolar macrophages. Lung lavage has been performed weekly for 6 wk in both species of monkey with no ill effects. This technique has many applications in the study of infection and pulmonary defense mechanisms. (Author)


Descriptors :   *LEUKOCYTES , *PHAGOCYTES , RECOVERY , EFFICIENCY , MONKEYS , ALVEOLI , MACACA FASCICULARIS , MACAQUE MONKEYS


Subject Categories : Anatomy and Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE