Accession Number : ADA043782


Title :   Radioimmunoassay of Urinary Free Cortisol.


Descriptive Note : Final rept.,


Corporate Author : NAVAL HEALTH RESEARCH CENTER SAN DIEGO CALIF


Personal Author(s) : Sokoloff,Roger L ; Hilderbrand,Richard L


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA043782


Report Date : 28 Sep 1976


Pagination or Media Count : 10


Abstract : The radioimmunoassay for urinary free cortisol described in this paper is simple, rapid, and reproducible. The method uses a commercially available antibody preparation and is performed in two steps. The first step includes an extraction and a column purification to remove materials antigenically similar to cortisol from the urine. The second step is the radioimmunoassay using dextran coated charcoal to separate bound and unbound cortisol. H3-cortisol is added prior to any mechanical manipulation to allow calculation of analytical recovery for the purification procedure. The coefficient of variation for interassay determinations was a maximum of 10.3% and for intra-assay determinations a maximum of 5.7%. Analytical recovery averaged 97.7%. One technician can analyze 100 samples per week. (Author)


Descriptors :   *URINE , *RADIOIMMUNOASSAY , *CORTISOL , DEXTRAN , ANTIBODIES , ABSORPTION , CHEMICAL ANALYSIS , SOLVENT EXTRACTION , COLUMN CHROMATOGRAPHY , CHARCOAL


Subject Categories : Biochemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE