Accession Number : ADA040120
Title : Electroimmunodiffusion Studies of alpha Chain, Secretory Piece and Secretory IgA.
Descriptive Note : Rept. for Mar 74-1977,
Corporate Author : ARMY INST OF DENTAL RESEARCH WASHINGTON D C
Personal Author(s) : Setterstrom,Jean A ; Gross,Arthur ; D'Alessandro,Sandra M
Report Date : 15 Mar 1977
Pagination or Media Count : 21
Abstract : Commercially available antisera to secretory piece, colostrum, and alpha chain were evaluated for use in electroimmunodiffusion of secretory IgA and secretory piece in biological fluids. Quantitation of secretory IgA presents difficulty since antisera to alpha chain indiscriminately precipitates both serum IgA and secretory IgA (11S) and production of antisera specific for antigenic determinants on secretory IgA is laborious and expensive. Problems in using currently available commercial antisera to colostrum and free secretory piece arise because they are multispecific. This report describes a technique modification to overcome that difficulty. Although monospecific antisera to I or P determinant would differentiate secretory piece or secretory IgA with one rocket precipitate, that same precipitate is identifiable in a multi-precipitate pattern by using a pure reference standard for establishment of a line of identity. The high level sensitivity of EID allows quantative assay of proteins in biological fluids collected in microliter amounts.
Descriptors : *IMMUNOASSAY , *IMMUNOGLOBULINS , QUANTITATIVE ANALYSIS , HUMANS , IDENTIFICATION SYSTEMS , LABORATORY PROCEDURES , ANTIBODIES , DIFFUSION , BODY FLUIDS , SEROLOGY , ELECTROPHORESIS , RABBITS , SECRETION , SALIVA , PRECIPITATES
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE