Accession Number : ADA033900


Title :   Toxicity of Polychlorinated Biphenyls to 'Euglena Gracilis': Cell Population Growth, Carbon Fixation, Chlorophyll Level, Oxygen Consumption, and Protein and Nucleic Acid Synthesis.


Descriptive Note : Scientific rept.,


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Ewald,W G ; French,J E ; Champ,M A


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA033900


Report Date : Jul 1976


Pagination or Media Count : 18


Abstract : Populations of Euglena gracilis in exponential growth under light were exposed to 2.5, 5.0, 7.5, and 10 ppm of Aroclor 1221. With Aroclor 1242, no inhibition of growth was observed with up to 100 ppm exposure. Cell cultures exposed to 4.4 ppm of Aroclor 1221 for 48 hours had a significantly reduced rate of carbon fixation and reduced levels of chlorophyll after correction for cell density. Oxygen consumption was not affected. Uptake of H(3)-leucine in treated cultures was twice that of controls, and H(3)-uridine uptake was significantly lower. Uptake of H(3)-thymidine, and incorporation of H(3)-leucine, H(3)-thymidine, and H(3)-uridine were not significantly different in treated and control cultures. An inexpensive and rapid screening method for potential toxic environmental chemical compounds used by military organizations is of great importance in case such compounds are accidently introduced into the environment.


Descriptors :   *WATER POLLUTION, *BIPHENYL, METHODOLOGY, TOXICITY, PROTEINS, INHIBITION, INDICATORS, BIOSYNTHESIS, NUCLEIC ACIDS, CHEMICAL AGENT DETECTORS, OXYGEN CONSUMPTION, GROWTH(PHYSIOLOGY), CHLOROPHYLLS, PHOTOSYNTHESIS


Subject Categories : Toxicology
      Water Pollution and Control


Distribution Statement : APPROVED FOR PUBLIC RELEASE