Accession Number : ADA033508
Title : Recovery, Structure and Function of Dog Granulocytes After Freeze Preservation with Dimethylsulfoxide.
Descriptive Note : Scientific rept.,
Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
Personal Author(s) : French,J. E. ; Flor,W. J. ; Grissom,M. P. ; Parker,J. L. ; Sajko,G.
Report Date : JUL 1976
Pagination or Media Count : 30
Abstract : The recovery, structure and function of dog granulocytes were determined before and after freeze preservation. Leucocytes were isolated from defibrinated or anticoagulated whole blood and subsequent erythrocyte sedimentation on a column of 2:1 dextran (6%)-Isopaque (33.9%). Granulocytes isolated by these procedures were examined for changes in O2 consumption associated with phagocytosis, in vitro directed migration (chemotaxis), bactericidal activity, and ultrastructure before and after freezing. Granulocytes were frozen in DMSO (7.5%) and autologous serum of HBSS and 20% autologous serum at the rate of -1 C/min to -80 C and stored in liquid N2 vapor. It is concluded that dog granulocytes freeze preserved by these methods are functional in vitro, but that phagocytic, directed migration, and bactericidal functions and ultrastructure are impaired to different degrees, according to the method of isolation and preparation for storage. These results indicate the need for continued investigation on the effects of storage variables on the preservation of granulocytes. The increased efficacy of granulocyte transfusions in conjunction with antibiotic therapy against microbial infection during agranulocytosis or granulocytopenia has been well documented. An effective method for granulocyte preservation would minimize logistical problems (typing, preservation, matching and transportation) in increasing the availability of this therapy to combat casualties. This model system was developed for studying granulocyte preservation methods.
Descriptors : *FREEZING, *GRANULOCYTES, SULFOXIDES, THAWING, STORAGE, PRESERVATION, DOGS, BIOASSAY, OXYGEN CONSUMPTION, CHEMOTAXIS, BLOOD PRESERVATIVES.
Subject Categories : ANATOMY AND PHYSIOLOGY
Distribution Statement : APPROVED FOR PUBLIC RELEASE