Accession Number : ADA033258
Title : Enhancement of 'In Vitro' Granulocyte-Macrophage Colony Formation by Normal Human Serum or Plasma
Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
Personal Author(s) : Weatherly, T. L.
Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=ADA033258
Report Date : AUG 1976
Pagination or Media Count : 19
Abstract : Irreversible bone marrow damage is the principal etiologic factor causing death after exposure to ionizing radiation. Enhancement of human and murine bone marrow colony formation in agar gel was studied with respect to species specificity, ability to enhance various colony stimulating factors, dose-response relationship, the abilities of nonhuman sera to enhance colony formation, and mechanism of action. Normal human serum enhanced colony formation by both human and murine marrow cells. Murine colony formation was enhanced by normal human serum when several different types of colony stimulation factors were used. Enhancement by nonhuman sera was absent or much less than by normal human sera. Preincubation of marrow with serum did not result in enhancement. Nonhuman colony stimulating factor did not decrease enhancement by normal human serum of human marrow colony formation.
Descriptors : *BONE MARROW , *IONIZING RADIATION , IN VITRO ANALYSIS , DOSAGE , MICE , BLOOD SERUM , GROWTH(PHYSIOLOGY) , CULTURES(BIOLOGY) , COLONIES(BIOLOGY) , LEUKOCYTES , HUMANS , STIMULATION(PHYSIOLOGY) , CONCENTRATION(CHEMISTRY)
Subject Categories : MEDICINE AND MEDICAL RESEARCH
Distribution Statement : APPROVED FOR PUBLIC RELEASE