Accession Number : ADA025272
Title : Serum Protein-Bound Hexosamine Analysis with Autoclave Hydrolysis.
Descriptive Note : Technical note,
Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
Personal Author(s) : Ryan,M J ; Routzahn,G E
Report Date : Mar 1976
Pagination or Media Count : 16
Abstract : A modification of the Winzler method for assay of serum protein-bound hexosamine is presented. The modified method eliminates the usual 4-hour hydrolysis period by substituting a 15-minute hydrolysis in an autoclave, thus allowing a more rapid processing of samples. No significant differences were found between assay values obtained with either method for a variety of samples. In addition, the concentration of the samples may be effectively doubled and the curve 'moved' to a more sensitive area of the spectrum without loss of sensitivity or accuracy.
Descriptors : *HYDROLYSIS , *CHEMICAL ANALYSIS , *BLOOD PROTEINS , SENSITIVITY , AMINES , BLOOD SERUM
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE