Accession Number : ADA025272


Title :   Serum Protein-Bound Hexosamine Analysis with Autoclave Hydrolysis.


Descriptive Note : Technical note,


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Ryan,M. J. ; Routzahn,G. E.


Report Date : MAR 1976


Pagination or Media Count : 16


Abstract : A modification of the Winzler method for assay of serum protein-bound hexosamine is presented. The modified method eliminates the usual 4-hour hydrolysis period by substituting a 15-minute hydrolysis in an autoclave, thus allowing a more rapid processing of samples. No significant differences were found between assay values obtained with either method for a variety of samples. In addition, the concentration of the samples may be effectively doubled and the curve 'moved' to a more sensitive area of the spectrum without loss of sensitivity or accuracy.


Descriptors :   *HYDROLYSIS, *CHEMICAL ANALYSIS, *BLOOD PROTEINS, SENSITIVITY, AMINES, BLOOD SERUM.


Subject Categories : BIOCHEMISRTY


Distribution Statement : APPROVED FOR PUBLIC RELEASE