Accession Number : ADA016191


Title :   Evaluation of Biologic Activity of Attenuated Endotoxin in Mice.


Descriptive Note : Scientific rept.,


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Galley,C. B. ; Walker,R. I. ; Ledney,G. D. ; Gambrill,M. R.


Report Date : JUN 1975


Pagination or Media Count : 24


Abstract : Bacterial endotoxins increase nonspecific host resistance to a number of stresses. For example, resistance to radiation-induced damage is achieved through stimulation of granulocytic proliferation by endotoxin. Endotoxin is also a B-lymphocyte mitogen and thus has been used as an adjuvant for promoting resistance to antigens such as cancer cell implants. Repeated small doses of endotoxin, furthermore, can be used to establish a refractory state (tolerance) to further challenge with endotoxin. All of the resistance enhancing actions of endotoxin described above may contribute to the ability of endotoxin to increase resistance to bacterial infections. Experiments were designed to test the hypothesis that Salmonella typhosa endotoxin could be attenuated by treatment with ferric chloride (FeCl3) and that this preparation retained the biological activity of normal endotoxin. The authors found attenuated endotoxin to be as biologically active as untreated endotoxin, but it had the additional advantage of being relatively nontoxic.


Descriptors :   *IMMUNOLOGY, *ENDOTOXINS, *RESISTANCE(BIOLOGY), EXPERIMENTAL DATA, BACTERIA, ATTENUATION, MICE, IMMUNITY, BACTERIAL DISEASES, BIOASSAY, LABORATORY ANIMALS, RADIOPROTECTIVE AGENTS.


Subject Categories : MEDICINE AND MEDICAL RESEARCH


Distribution Statement : APPROVED FOR PUBLIC RELEASE