Accession Number : ADA016190


Title :   The Influence of a Granulocytic Inhibitor (Chalone) on Hematopoiesis in an 'in vivo' Culture System.


Descriptive Note : Scientific rept.,


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : MacVittie,T. J. ; McCarthy,K. F. ; McCarthy,E. G.


Report Date : JUN 1975


Pagination or Media Count : 22


Abstract : The in vivo diffusion chamber (DC) technique for mouse marrow culture was used to determine the effect of a granulocyte inhibitor (chalone) on the proliferation of the pluripotent stem cell (CFU-S) and the granulocyte progenitor cell (CFU-C). The DC's were each inoculated with a normal mouse marrow suspension and then implanted into host mice exposed four hours previously to 900 rads 60Cogamma-radiation. Granulocyte inhibitor (chalone) containing solution was collected as the 20-hour incubation media of rat granulocytes previously obtained by peritoneal lavage. The granulocytic inhibitor was injected intraperitoneally in 1.5-ml aliquots into chamber-bearing mice at 5, 20, 24, 28, 44 and 48 hours after implanation. This regimen resulted in a significantly reduced number of granulocytic progeny formed within the DC's while there was no growth inhibition of mouse fibroblast cultured under identical conditons. The reduced cell production was due in part to a significant reduction in the self-renewal rate of the CFU-C while no apparent direct effect was observed upon the growth of the CFU-S within the same cultures.


Descriptors :   *GRANULOCYTES, *BLOOD CELLS, IN VITRO ANALYSIS, INHIBITORS, IN VIVO ANALYSIS, CULTURES(BIOLOGY), BONE MARROW, MITOSIS.


Subject Categories : ANATOMY AND PHYSIOLOGY


Distribution Statement : APPROVED FOR PUBLIC RELEASE