Accession Number : ADA012639
Title : Gamma-Aminobutyric Acid Uptake and Metabolism in 'Aplysia dactylomela'
Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
Personal Author(s) : Zeman, G H ; Myers, P R ; Dalton, T K
Report Date : Apr 1975
Pagination or Media Count : 35
Abstract : Selected ganglia of Aplysia dactylomela were found to possess a sodium dependent gamma-aminobutyric acid (GABA) uptake system. Tissue to medium ratios as high as 20:1 were calculated in the buccal ganglion after a 45-minute incubation in 6 Micromoles GABA. The metabolism of accumulated GABA was low with 90 percent of the radioactivity remaining as GABA after a 45-minute incubation and 20-minute washout period. The buccal ganglion displayed a sodium dependent, high affinity uptake system with a Km = 52 Micromoles. No net sodium dependent accumulation was found in the pedal ganglion. In the buccal ganglion, light microscope autoradiography indicated that the sites of GABA accumulation were predominantly extraneuronal.
Descriptors : *GANGLIA , *SPONGES , ACCUMULATION , BIOCHEMISTRY , BIOLOGICAL ABSORPTION , BUTYRIC ACIDS , METABOLISM , TISSUES(BIOLOGY)
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE