Accession Number : ADA001675


Title :   Morphine-Induced Changes in the Activity of Enzymes Involved in Neutrotransmitter Metabolism in Specific Brain Regions of the Tolerant Rat,


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Catravas,G. N. ; McHale,C. G. ; Cohan,S. L.


Report Date : MAY 1974


Pagination or Media Count : 23


Abstract : The activities of choline acetyl transferase (E.C.2.3.1.6.), acetylcholinesterase (E.C.3.1.1.7.) and monoamine oxidase (E.C.1.4.3.4.) were determined in the hippocampus, thalamus, hypothalamus, cerebral cortex and basal ganglia of groups of rats sacrificed at predetermined times following the last scheduled morphine injection. It was found that, in animals that showed a paradoxical activation reaction shortly after the last morphine injection ('jumping' rats), monoamine oxidase activity decreased in all brain areas studied with lowest values between approximately 30 and 60 minutes, and returned to nearly normal values by six hours postinjection. Acetylcholinesterase activity showed oscillations above control values. Oscillating changes were also observed in the activity of choline acetyl transferase depending on the time of sacrifice of the animal. No appreciable changes in monoamine oxidase activity were observed in 'nonjumping' tolerant rats.


Descriptors :   *ENZYMES, *NEUROCHEMISTRY, *MORPHINE, EXPERIMENTAL DATA, BRAIN, RATS, METABOLISM, ACETYLCHOLINESTERASE, PHARMACOLOGY, MONOAMINE OXIDASE, TRANSFERASES.


Subject Categories : PHARMACOLOGY


Distribution Statement : APPROVED FOR PUBLIC RELEASE