Accession Number : AD1027869

Title :   Array-Based Discovery of Aptamer Pairs

Descriptive Note : Journal Article

Corporate Author : University of California - Santa Barbara Santa Barbara United States

Personal Author(s) : Oh,Seung S ; Nie,Jeff ; Stewart,Ron ; Radeke,Monte J ; Eisenstein,Michael ; Coffey,Peter J ; Thomson,James A ; Soh,H T ; Cho,Minseon

Full Text :

Report Date : 11 Dec 2014

Pagination or Media Count : 11

Abstract : Affinity reagent pairs that recognize distinct epitopes on a target protein can greatly improve the sensitivity and specificity of molecular detection. Importantly, such pairs can be conjugated to generate reagents that achieve two site bidentate target recognition, with affinities greatly exceeding either monovalent component. DNA aptamers are especially well-suited for such constructs, because they can be linked via standard synthesis techniques without requiring chemical conjugation. Unfortunately, aptamer pairs are difficult to generate, primarily because conventional selection methods preferentially yield aptamers that recognize a dominant hot spot epitope. Our array-based discovery platform for multivalent aptamers (AD-MAP) overcomes this problem to achieve efficient discovery of aptamer pairs. We use microfluidic selection and high-throughput sequencing to obtain an enriched pool of aptamer sequences. Next, we synthesize a custom array based on these sequences, and perform parallel affinity measurements to identify the highest-affinity aptamer for the target protein.

Descriptors :   sequences , detection , molecules , nucleotides , dna microarrays

Subject Categories : Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE