Accession Number : AD1007412


Title :   Subgrouping Chronic Fatigue Syndrome Patients By Genetic and Immune Profiling


Descriptive Note : Technical Report,08 Sep 2012,07 Sep 2015


Corporate Author : The Leland Stanford Junior University Menlo Park United States


Personal Author(s) : Montoya,Jose ; Holmes,Tyson ; Gavert,Donn ; Maecker,Holden ; Mindrinos,Michael ; Fernandez,Rosemary ; Mendiola,Luciana


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=AD1007412


Report Date : 01 Dec 2015


Pagination or Media Count : 185


Abstract : We have successfully performed all testing of the approximately 600 samples (200 CFS/ME patients vs 397 healthy controls) we set up to do under this grant at the Stanford Human Immune Monitoring Center (HIMC) and the Stanford HLA Mindrinos lab. These samples were tested by two novel methods (CyTOF-phosphoflow and HLA Typing, respectively) to help us better understand the roles of immune responses and genetics in the pathogenesis of chronic fatigue syndrome (CFS)/myalgic encephalomyelitis (ME). It is likely that these findings will also result in an improved understanding of the pathogenesis of Gulf War Illness(GWI). For both methods, CyTOF-phosphoflow and HLA Typing, we have reviewed in excruciating detail all the potential sources of technical error in both methods, systematically cleaned all confounders and plate effects, and have set up a statistical plan.


Descriptors :   CHRONIC FATIGUE SYNDROME , AUTOIMMUNE DISEASES , DYNAMIC RANGE , quality control , genetics , immune system , INFECTIOUS DISEASES , GULF WAR SYNDROME


Distribution Statement : APPROVED FOR PUBLIC RELEASE