Accession Number : AD0783023


Title :   Graft versus Host Disease in Rats after Donor Treatment with Cyclophosphamide and Spleen Cells of Host Origin.


Descriptive Note : Scientific rept.,


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Fink,M. P. ; Cloud,C. L. ; Exum,E. D.


Report Date : APR 1974


Pagination or Media Count : 20


Abstract : Lethally irradiated Lewis-Brown Norway F1 hybrid (LBN) rats were grafted with either marrow or marrow and spleen cells from normal Lewis (L) donors. All recipients of inocula containing both marrow and spleen cells died of graft versus host (GVH) disease. In other irradiated and grafted rats, the severity of the GVH disease was reduced by challenge of the donor with LBN spleen cells prior to or after treatment with cyclophosphamide (CY). The magnitude of this suppressed immunity depended on the interval between the administration of CY and the allogeneic spleen cells. Maximum suppression was obtained when donors received CY on day 0 and LBN cells on day +1. In all experiments, the treated donors were sacrificed on day +7. The observed immunosuppression was shown to result from the synergistic action of CY and cells bearing the alloantigens of the recipient. Furthermore, the phenomenon was demonstrated to be antigen specific. (Author)


Descriptors :   *Surgical transplantation, *Immunosuppression, Irradiation, Organic phosphorus compounds, Rats, Hosts(Biology), Spleen, Immunology, Bone marrow, Radiobiology, Drugs


Subject Categories : RADIOBIOLOGY
      PHARMACOLOGY


Distribution Statement : APPROVED FOR PUBLIC RELEASE