Accession Number : AD0763351


Title :   Separation of Spleen Cells in Iso-Osmotic Ficoll Density Gradients.


Descriptive Note : Scientific rept.,


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : McCarthy,K. F. ; Nutter,J. E. ; MacVittie,T. J.


Report Date : MAR 1973


Pagination or Media Count : 25


Abstract : Osmotic pressures of polymer solutions were examined as a function of density by equilibrium dialysis, and it was reaffirmed that the van't Hoff equation cannot be used to calculate the osmotic pressure of polymer solutions at concentrations greater than 5 percent. Therefore, it appears that previous methods utilizing polymer solutions for the generation of density gradients also generated hyperosmotic gradients. However, since cells maintain their integrity best in an isotonic solution, an iso-osmotic density gradient can be prepared by superimposing a reverse salt gradient on a Ficoll gradient. Using this method, the density profiles of several cell populations were determined, and observations on fractionated C57BL mouse spleen were made. (Modified author abstract)


Descriptors :   (*SPLEEN, CELLS(BIOLOGY)), (*CELLS(BIOLOGY), DENSITY), BIOPHYSICS, OSMOTIC PRESSURE, IMMUNOLOGY


Subject Categories : MEDICINE AND MEDICAL RESEARCH


Distribution Statement : APPROVED FOR PUBLIC RELEASE