Accession Number : AD0745747


Title :   Determination of L-Fucose in Glycoproteins. I. Effect of Nonfucose Moieties of Serum Glycoproteins,


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Sobocinski,P. Z. ; Canterbury,W. J. ; Hartley,K. M.


Report Date : APR 1972


Pagination or Media Count : 33


Abstract : The effect of galactose, mannose, and tryptophan on the determination of the methylpentose L-fucose in glycoproteins by the cysteine-sulfuric acid reaction is described. Under certain analytical conditions, protein-bound hexoses significantly contribute to the apparent fucose level. The true fucose level may be approximated under these same conditions by means of an empirically derived equation relating the concentration of serum protein-bound hexoses to the true fucose level. Fucose levels obtained with pathological and nonpathological sera by various methods are compared and specific conditions for the reliability of the statistics are defined and discussed. (Author)


Descriptors :   (*GLYCOPROTEINS, FRUCTOSES), (*FRUCTOSES, BLOOD CHEMISTRY), BLOOD SERUM, DISEASES, DIAGNOSIS(MEDICINE), CHEMICAL ANALYSIS


Subject Categories : BIOCHEMISRTY


Distribution Statement : APPROVED FOR PUBLIC RELEASE