Accession Number : AD0724295


Title :   Acetylcholinesterase Activity in Synaptic Vesicles of the Medulla of Rats Exposed to High Doses of Gamma-Neutron Radiation


Descriptive Note : Scientific rept.


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Darden, J H ; Rene, A A ; Parker, J L


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD= AD0724295


Report Date : Apr 1971


Pagination or Media Count : 21


Abstract : Sprague-Dawley rats were subjected to a whole-body dose of 15 krads of mixed gamma-neutron radiation. At 2 and 24 hours following irradiation 14 animals from the irradiated group and 14 unirradiated controls were sacrificed by replacing the blood in the brain circulation by perfusion with a sucrose- eserine solution. The medulla section of the brain was removed, homogenized and prepared for ultracentrifugation and sucrose density gradient separation. The separated synaptic vesicles were assayed for protein and acetylcholinesterase (ACHE). A decrease in protein was found 2 hours after irradiation with a greater decrease 22 hours later. A decrease in ACHE activity was also seen 2 hours after irradiation but there was no further decrease at 24 hours. These results were correlated with vascular changes found during the central nervous system (CNS) syndrome.


Descriptors :   *ACETYLCHOLINESTERASE , *BRAIN , *RADIATION EFFECTS , CENTRAL NERVOUS SYSTEM , ENZYMES , GAMMA RAYS , NERVE IMPULSES , NERVOUS SYSTEM , NUCLEAR RADIATION , PHYSIOLOGY , PROTEINS


Subject Categories : Biochemistry
      Anatomy and Physiology
      Radiobiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE