Accession Number : AD0715474


Title :   Radiation-Induced Ultrastructural Changes in Lysosomes. II. Biochemical Analysis.


Descriptive Note : Scientific rept.,


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Rene,A. A. ; Darden,J. H. ; Parker,J. L.


Report Date : AUG 1970


Pagination or Media Count : 21


Abstract : A biochemical analysis for acid phosphatase was made of soluble protein and lysosomes in liver cells of rats exposed to 2 krads of x rays. The results clearly showed an increase in the acid phosphatase activity in the lysosomes and in soluble protein 2 hours after exposure. This was followed by a decrease in enzyme activity in the lysosomes and a consistent high level in the soluble protein. The results correlated well with earlier cytochemical studies and showed that, in addition to the radiation-induced increase in number and size of lysosomes, there is a distinct increase of acid phosphatase in the lysosomes themselves. This substantiated earlier findings. In addition, the persistent high level of acid phosphatase in the soluble protein (cytoplasm) was a possible indication of early radiation impairment of the lysosomal membrane. (Author)


Descriptors :   (*RADIATION EFFECTS, *CELL STRUCTURE), CELLS(BIOLOGY), BIOCHEMISTRY, ENZYMES, MEMBRANES(BIOLOGY), NECROSIS, RATS, LIVER, PHOSPHORIC MONOESTER HYDROLASES, PROTEINS, CORRELATION TECHNIQUES


Subject Categories : ANATOMY AND PHYSIOLOGY
      RADIOBIOLOGY


Distribution Statement : APPROVED FOR PUBLIC RELEASE