Accession Number : AD0709942


Title :   VIRUS-SPECIFIC POLYSOMES IN CELLS INFECTED WITH THE VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS,


Corporate Author : FORT DETRICK FREDERICK MD


Personal Author(s) : Ershov,F. I. ; Zhdanov,V. M. ; Uryvaev,L. V.


Report Date : 09 JUL 1970


Pagination or Media Count : 9


Abstract : The use of a double isotopic tag is a convenient methodological technique which permits simultaneous determination of the structural state and the functional activity of the polysomes. Study of the dynamics of polysome formation in the case of arbovirus infection testifies to the fact that this process is closely correlated with the virus reproduction cycle. In the case of infection by the VEE virus, the maximum of the formation of actively functioning virus-specific polysomes is noted between the 3-rd and the 4-th hour after infection, i.e., at the height of the infection. Of the 2 types of virus-specific polysomes the greatest interest is afforded by 'heavy' polysomes, which have a sedimentation constant of 250 S and higher. They are, apparently, the main structure on the basis of which the synthesis of virus proteins is effected. A special communication will be devoted to a detailed analysis of the state and functioning of virus-specific polysomes of this type. The presence, on the polysomes, of an RNA which in its properties is analogous to that of the viri, apparently testifies to its information functions. (Author)


Descriptors :   *VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS, *RIBOSOMES, *TISSUE CULTURE CELLS, RIBOSOMES, GROWTH(PHYSIOLOGY), INFECTIOUS DISEASES, ARBOVIRUSES, VIRUSES, NUCLEIC ACIDS, BIOSYNTHESIS, USSR, MOLECULAR STRUCTURE.


Subject Categories : BIOCHEMISRTY
      MICROBIOLOGY


Distribution Statement : APPROVED FOR PUBLIC RELEASE