Accession Number : AD0701756


Title :   RADIATION-INDUCED ULTRASTRUCTURAL CHANGES IN LYSOSOMES. I. CYTOCHEMICAL ANALYSIS.


Descriptive Note : Scientific rept.,


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Rene,A. A. ; Parker,J. L. ; Darden,J. H.


Report Date : DEC 1969


Pagination or Media Count : 27


Abstract : Ultrastructural and biochemical changes in lysosomes of rat liver following exposure to ionizing radiation were studied. A marker for acid phosphatase was used to visually correlate the progressive changes in lysosomes with the cellular necrobiotic process postirradiation. The earliest observable change in the lysosomes and/or lysosomal enzymes corresponding with the sequence of fine structural alterations following irradiation suggests that radiation labilizes the lysosomal membrane resulting in a release of enzymes responsible for cell damage. The concentration of the lead phosphate reaction product indicated that the initial action on the lysosome is evidently a 'build-up' of hydrolytic enzymes within 2 hours after irradiation followed by a gradual release of the marked enzyme 2-24 hours postirradiation as noted by decreased enzyme concentration within the lysosomes. The release of the enzyme appeared to be directly related to an increasing cellular necrobiosis following irradiation. (Author)


Descriptors :   (*RADIATION EFFECTS, *CELL STRUCTURE), CELLS(BIOLOGY), ENZYMES, HYDROLYSIS, DAMAGE, NECROSIS, BIOCHEMISTRY, MORPHOLOGY(BIOLOGY), LIVER, RATS, ELECTRON MICROSCOPY, HISTOLOGY


Subject Categories : ANATOMY AND PHYSIOLOGY
      RADIOBIOLOGY


Distribution Statement : APPROVED FOR PUBLIC RELEASE