Accession Number : AD0701756


Title :   RADIATION-INDUCED ULTRASTRUCTURAL CHANGES IN LYSOSOMES. I. CYTOCHEMICAL ANALYSIS


Descriptive Note : Scientific rept.


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Rene, A A ; Parker, J L ; Darden, J H


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD= AD0701756


Report Date : Dec 1969


Pagination or Media Count : 31


Abstract : Ultrastructural and biochemical changes in lysosomes of rat liver following exposure to ionizing radiation were studied. A marker for acid phosphatase was used to visually correlate the progressive changes in lysosomes with the cellular necrobiotic process postirradiation. The earliest observable change in the lysosomes and/or lysosomal enzymes corresponding with the sequence of fine structural alterations following irradiation suggests that radiation labilizes the lysosomal membrane resulting in a release of enzymes responsible for cell damage. The concentration of the lead phosphate reaction product indicated that the initial action on the lysosome is evidently a 'build-up' of hydrolytic enzymes within 2 hours after irradiation followed by a gradual release of the marked enzyme 2-24 hours postirradiation as noted by decreased enzyme concentration within the lysosomes. The release of the enzyme appeared to be directly related to an increasing cellular necrobiosis following irradiation.


Descriptors :   *CELL STRUCTURE , *RADIATION EFFECTS , BIOCHEMISTRY , CELLS(BIOLOGY) , DAMAGE , ELECTRON MICROSCOPY , ENZYMES , HISTOLOGY , HYDROLYSIS , LIVER , MORPHOLOGY(BIOLOGY) , NECROSIS , RATS


Subject Categories : Anatomy and Physiology
      Radiobiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE