Accession Number : AD0695423


Title :   RESPONSE OF RAT GASTROINTESTINAL TRACT DEHYDROGENASE SYSTEMS TO WHOLE-BODY IONIZING IRRADIATION


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Kivy-Rosenberg, E.


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=AD0695423


Report Date : OCT 1969


Pagination or Media Count : 18


Abstract : The activity of two dehydrogenase systems of the pentose cycle (glucose-6-phosphate-dependent and 6-phosphogluconate-dependent) as well as the lactate-dependent dehydrogenase system was studied in homogenates of four regions of the adult male rat gastrointestinal tract following 1540-rad whole- body x irradiation (WBR), or 1400-rad mixed gamma-neutron radiation. Microchemical assays using a tetrazolium salt (INT) were done at intervals after irradiation (10-20 minutes, 1, 2, 3 days). By 1 day postirradiation there was a significant fall in activity in both pentose cycle systems as well as in the lactate-dependent system in the stomach. For the most part, the fall in activity did not occur in the intestinal regions until the 2nd day. This depression became most prominent by 3 days for the regions studied. The lactate-dependent dehydrogenase system activity of three regions of the gastrointestinal tract was more drastically affected by the radiations delivered than was that of the pentose cycle systems. The fourth region (distal end of the large intestine) was not consistent with the others, in its response. It seems from this study that the dehydrogenase systems which are intramitochondrial in location showed a greater reduction in activity than did those which are extramitochondrial.


Descriptors :   *WHOLE BODY IRRADIATION, *ENZYMES, *RADIATION EFFECTS, RESPONSE(BIOLOGY), INHIBITION, PENTOSES, RADIATION DOSAGE, GASTROINTESTINAL SYSTEM, LACTATES, OXIDOREDUCTASES, BIOCHEMISTRY, ADSORPTION, RATS, INTESTINES


Subject Categories : BIOCHEMISRTY
      RADIOBIOLOGY


Distribution Statement : APPROVED FOR PUBLIC RELEASE