Accession Number : AD0689097


Title :   PURIFICATION OF RAT GAMMA GLOBULIN AND THE PRODUCTION OF A SPECIFIC ANTI-RAT GAMMA GLOBULIN SERUM


Descriptive Note : Technical note


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Exum, E D ; Bowser, R T


Full Text : http://www.dtic.mil/get-tr-doc/pdf?AD=AD0689097


Report Date : Mar 1969


Pagination or Media Count : 24


Abstract : A method for the isolation of rat 7S gamma globulin based on multiple adsorptions with DEAE-Sephadex A-50 has been presented. The separation of rat 7S globulin requires a procedure modified from the one used for humans in terms of the buffer pH, and the number of adsorptions. Ultracentrifugal analysis indicates the resulting fraction is a single component. In addition, immunoelectrophoresis of the fraction when tested against anti-rat sera confirms the homogeneity of this 7S fraction. A monospecific anti-serum to rat 7S gamma globulin has been produced. This antiserum is free of reaction with all other serum components when subjected to immunoelectrophoresis against normal rat serum.


Descriptors :   *GAMMA GLOBULIN, *IMMUNE SERUMS, ADSORPTION, BLOOD SERUM, BUFFERS, ELECTROPHORESIS, PH FACTOR, PREPARATION, PURIFICATION, RATS


Subject Categories : Biochemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE