Accession Number : AD0689097


Title :   PURIFICATION OF RAT GAMMA GLOBULIN AND THE PRODUCTION OF A SPECIFIC ANTI-RAT GAMMA GLOBULIN SERUM.


Descriptive Note : Technical note,


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Exum,E. D. ; Bowser,R. T.


Report Date : MAR 1969


Pagination or Media Count : 21


Abstract : A method for the isolation of rat 7S gamma globulin based on multiple adsorptions with DEAE-Sephadex A-50 has been presented. The separation of rat 7S globulin requires a procedure modified from the one used for humans in terms of the buffer pH, and the number of adsorptions. Ultracentrifugal analysis indicates the resulting fraction is a single component. In addition, immunoelectrophoresis of the fraction when tested against anti-rat sera confirms the homogeneity of this 7S fraction. A monospecific anti-serum to rat 7S gamma globulin has been produced. This antiserum is free of reaction with all other serum components when subjected to immunoelectrophoresis against normal rat serum. (Author)


Descriptors :   (*IMMUNE SERUMS, PREPARATION), (*GAMMA GLOBULIN, PURIFICATION), ADSORPTION, ELECTROPHORESIS, BUFFERS, PH FACTOR, BLOOD SERUM, RATS


Subject Categories : BIOCHEMISRTY


Distribution Statement : APPROVED FOR PUBLIC RELEASE