Accession Number : AD0622341


Title :   A FLUOROMETRIC PROCEDURE FOR MEASURING THE ACTIVITY OF DEHYDROGENASES.


Descriptive Note : Rept. for Jul 64-Jan 65,


Corporate Author : CHEMICAL RESEARCH AND DEVELOPMENT LABS EDGEWOOD ARSENAL MD


Personal Author(s) : Guilbault,George G. ; Kramer,David N.


Report Date : AUG 1965


Pagination or Media Count : 16


Abstract : A simple, direct, fluorometric method is described for measuring the activity of dehydrogenases. The method is based upon the conversion of the nonfluorescent material resazurin to the highly fluorescent compound resorufin, in conjunction with the nicotinamide adenine dinucleotide-reduced nicotinamide adenine dinucleotide (NAD(+)-NADH) system. By the procedures described, approximately 0.00010 to 0.1000 unit/ml of lactic acid dehydrogenase, alcohol dehydrogenase, malic acid dehydrogenase, glutamic acid dehydrogenase, glucose6-phosphate dehydrogenase, L-alphs-glycero phosphate dehydrogenase (GPDH), and glycerol dehydrogenase (GDH) may be determined with standard deviations of approximately =1.0%. Similarly, diaphorase, 0.000400 to 0.0800 unit/ml, resazurin, 0.00000001 to 0.00001 M, and NADH, 0.0000002 to 0.00002 M, may be determined with standard deviations of =0.5%, =0.8%, and =0.5%, respectively. (Author)


Descriptors :   (*OXIDOREDUCTASES, CHEMICAL ANALYSIS), FLUORESCENCE, DYES, CHEMICAL INDICATORS, NUCLEODIDES, NITROGEN HETEROCYCLIC COMPOUNDS, OXYGEN HETEROCYCLIC COMPOUNDS


Distribution Statement : APPROVED FOR PUBLIC RELEASE